Table 1.
Best-fit viral kinetic parameters for WT and MUT H1N1pdm09a
| Parameter | Value (95% CI) |
P valueb | |
|---|---|---|---|
| WT | MUT | ||
| Mean eclipse period, τE (h) | 6.6 (5.8–7.7) | 9.1 (7.8–11.6) | 0.013 |
| Eclipse period SD, σE (h) | 1.2 (0.81–1.8) | 1.6 (0.88–2.8) | 0.49 |
| Infecting time, tinfect (min) | 31 (20–47) | 22 (11–38) | 0.35 |
| MDCKα2,6 infectious life span, τI (h) | 49 (39–57) | 41 (30–50) | 0.24 |
| Virion infectious half-life, ln2/c (h) | 5.2 | 5.2 | |
| Production rate/cell, pRNA (no. of RNA copies/h) | 2,200 (960–4,600) | 370 (130–960) | 0.004 |
| Viral burst size, b = pRNA × τI (no. of RNA copies) | 110 (47–220) × 103 | 15 (5.3–37) × 103 | 0.001 |
| Basic reproductive no., R0 | 1.7 (0.66–4.3) × 103 | 3.0 (0.82–12) × 103 | 0.51 |
| No. of infectious units per particle, R0/b | 16 (3.8–68) × 10−3 | 190 (36–1,000) × 10−3 | 0.031 |
a
Fitted parameter values for WT and MUT H1N1pdm09 with 95% CIs from bootstrap replicates of SC and MC viral yield experiments.
b
WT versus MUT.