Restriction endonuclease digestion of Stx bacteriophage DNA. Restriction nuclease-digested bacteriophage DNA separated on 0.7% agarose digested with EcoNI (lanes 1 to 5), BsrBI (lanes 6 to 10), and PvuI (lanes 11 to 15). Lanes 1, 6, and 11, phage P8983; 2, 7, and 12, phage P13344; 3, 8, and 13, phage P13374; 4, 9, and 14, phage P13771; and 5, 10, and 15, phage P13772. M, molecular size standard (range, 0.2 to 10 kb).