Table 2.
PCR primers for detection of the wrbA gene, the Stx2 phage integration site into wrbA, and circular phage DNA
| Gene and/or integration site | GenBank accession no. | Position in sequence | Primer | Nucleotide sequence (5′–3′) | Melting temp (°C) | PCR product (bp) |
|---|---|---|---|---|---|---|
| wrbA; integrase prophage P13374 (ORF01) | AE005174 | 1,330,538–1,330,556 | ML1 | GTGGAACTAAAGACGCTCG | 55.0 | 500 |
| 1,331,037–1,331,016 | ML2 | ATTTATTGCATCACAGATGGGG | ||||
| wrbA; prophage P13374 (ORF79) | HE664024 | 60,619–60,638 | orf79 | ACGGTAAATACCCTACCCAG | 50.8 | 314 |
| AP009048 | 1,068,130–1,068,108 | wrbA-R | ATGGCTAAAGTTCTGGTGCTTTA | |||
| wrbA | AP009048 | 1,067,534–1,067,550 | wrbA-F | TTAGCCGTTAAGTTTAACTGCC | 57 | 596 |
| 1,068,130–1,068,108 | wrbA-R | ATGGCTAAAGTTCTGGTGCTTTA | ||||
| Prophage P13374 (ORF79); integrase prophage P13374 (ORF01) | HE664024 | 60,619–60,638 | orf79 | ACGGTAAATACCCTACCCAG | 50.8 | 474 |
| 190–210 | ML2 | ATTTATTGCATCACAGATGGGG |