Table 4.
Peptide identification by PMF and PFF using MALDI-TOF-TOF MS/MS and MASCOT database searching on tryptic digests of P13374 proteins
| P13374 ORF | Nucleotide position | Molecular mass (kDa) |
No. of peptides found (method used) | GenBank accession no. (GI) | Function, name | |
|---|---|---|---|---|---|---|
| Calculated | Estimateda | |||||
| 58 | 34,002–36,146 | 81.030 | 70–90 | 11 (PMF) | EGR59966 (340730675) | Putative portal protein (Escherichia coli O104:H4 strain 01-09591) |
| 60 | 37,335–38,549 | 44.877 | 46–50 | 28 (PMF); 5 (PFF) | NP_049514 (9632520) | Hypothetical protein 933Wp54 (Enterobacteria phage 933W) |
| 61 | 38,605–38,994 | 13.496 | <14 | 7 (PMF); 2 (PFF) | NP_049515 (9632521) | Hypothetical protein 933Wp55 (Enterobacteria phage 933W) |
| 78 | 51,952–60,372 | 309.315 | >250 | 3 (PFF) | NP_049532 (9632538) | Hypothetical protein 933Wp72 (Enterobacteria phage 933W) |
a
Data are from SDS-PAGE analysis.