Fig 2.

FN siRNA inhibits H1N1, but not H5N1 virus replication. A549 cells with mock transfection and cells treated with FN, scrambled, or GAPDH siRNA molecules were incubated with influenza virus at an MOI of 1. Cells were fixed at 24 h postinfection and stained for M2 protein (green) and nuclei (blue). The percentages of infected cells (i.e., M2 positive) were deduced from five random views. The samples were examined on a fluorescence microscope and photographed at a ×400 magnification.