Fig 5.

Proteins encoded by ORF2 and ORF4 cooperate for the production of infectious VLPs. BSR-T7 cells were transfected with the NYMV minigenome encoding Gaussia luciferase and the indicated pCA expression vectors. Supernatants were collected at 72 h posttransfection and clarified through a 0.45-μm filter. Five hundred microliters of the clarified supernatants were transferred onto 293T cells previously transfected with pCA-N, pCA-ORF3, and pCA-L. Forty-eight hours later, the 293T cells were lysed and Gaussia-mediated light emission was quantified. The value obtained for the negative control (receiving only pCA-N, -ORF3, and -L) (lane 1) was arbitrarily set to 1. Bars represent the average values of three independent experiments. Standard deviations are shown. RLU = relative light units.