Fig 7.

NYMV RNA is a poor inducer of IFN-β. Five hundred nanograms of RNA isolated from purified viral particles (A) or infected cells (B) was transfected into 293T cells that were transfected with a plasmid coding for firefly luciferase under the control of the IFN-β promoter. Where indicated, the viral RNA was treated with shrimp alkaline phosphatase (SAP) prior to transfection. Firefly luciferase activity was normalized for transfection efficacy, and the value for the mock control was arbitrarily set to 1. Bars represent the average values of three independent experiments. Standard deviations are shown. RLU = relative light units.