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Schematic representation of Ad5 polymerase domain organization and location of conserved residues. (A) Domain organization of Ad5 Pol. Shaded regions represent conserved domains; regions shaded in black (and identified in boldface type) represent the conserved motifs that underwent mutagenesis in this study ([I/Y]xGG, motif A, and motif B). (B) Multiple-sequence alignment of conserved domains in the Ad5, Phi29, and Pfu DNA polymerases.
