Fig 5.

The I664V Ad5 mutant is selectively cytotoxic for cancer cells but not normal cells. (A and B) BJ (A) and HCA-2 (B) normal foreskin fibroblast cells were infected with either the WT or the I664V Ad5 mutant at an MOI of 20, and cell killing was measured 7 days later. (C to E) Various cancer cell lines (H1299 [C], HeLa [D], SF539 [E], and MCF-7 [F]) were infected with the WT or the I664V Ad5 mutant at an MOI of 5, and cell killing was measured 7 days later. Data represent mean values of 6 technical replicates; error bars denote the standard deviations. * indicates a P value of <0.01 and ** indicates a P value of <0.001; n.s. indicates values that were not statistically significantly different, as determined by a one-way analysis of variance with the Bonferroni post hoc test.