Fig 2.

Neutrophils do not play a role in restricting RSV replication in naïve mice or in the restriction of RSV in vivo by RSV-specific antibodies. The plan of each experiment is shown on the left, and the results are shown on the right. (A) Neutrophils do not restrict pulmonary RSV replication in RSV-naïve mice. BALB/c mice in groups of 5 were treated with neutrophil-specific antiserum (anti-PMN IP) or normal serum and infected with mG RSV. Five animals from each group were sacrificed on day 3 to quantify pulmonary neutrophils by flow cytometry. The remaining animals were sacrificed on day 4, and the pulmonary RSV titers were determined (PFU/g, mean ± SE). (B) Neutrophils do not affect the ability of RSV-specific antibodies to restrict RSV in vivo. Mice in groups of 5 were treated with anti-PMN antiserum or mock treated, treated with RSV F-specific antiserum or mock treated, and infected with mG RSV. The animals were sacrificed on day 4, and the pulmonary RSV titers were determined (PFU/g, mean ± SE). Statistical significances by Student's unpaired t test: groups AB, P = 0.012; groups AC, P = 0.006.