Fig 4.

CD8⁺ CD103⁺ DC are not inherently defective in the ability to stimulate proliferation and the acquisition of IFN-γ production in naïve CD8⁺ T cells. Mice received 10 μg poly(I·C) by intratracheal instillation. Twenty-four hours later, MLN cells were isolated, divided, and either pulsed with limiting 10⁻¹¹ M Ova peptide (a concentration that relies on the presence of mature APC for activation) or left untreated. DC subsets of interest were isolated by sorting followed by culture with CFSE-labeled OT-I T cells. At the completion of the 3-day culture period, cells were restimulated with peptide and CFSE dilution and IFN-γ production was measured. Representative data are shown (A). Averaged data for proliferation (B) and IFN-γ production (C). The background for IFN-γ production was determined using OT-I T cells that had been cultured with non-peptide-pulsed DC. Data are derived from three independent experiments. Error bars represent the standard errors of the means (SEM). Significance was determined using Student's t test. ns, not significant.