Fig 2.

Differences in viral degradation are not solely responsible for modulation of HIV-1 internalization in activated MDMs. MDMs were first differentiated for 7 days; either left untreated (M0), treated with IFN-γ (20 ng/ml) and LPS (100 ng/ml) (M1), or treated with IL-4 (20 ng/ml) (M2a) for 72 h; washed; and then pretreated or not with bafilomycin A1 (final concentration of 100 nM) for 30 min. Cells were than allowed to internalize equal amounts of NL4-3Balenv virus (standardized in terms of p24), in the presence of the drug, for 2.5 h at 37°C. After a 5-min trypsin treatment to remove membrane-bound viruses, cell lysis was performed. The total intracellular p24 content was quantified by an ELISA and normalized for cell viability (Cellular viability test on uninfected cells). Results are expressed as fold changes compared to M0 for M1-activated (top) and M2a-activated (bottom) MDMs and represent mean values (triplicate samples) of several independent experiments. In each graph, one symbol represents MDMs from one specific blood donor.