Fig 2.

TTSuV1a or TTSuV1b serum WB and ELISA. (A) WB analyses using gnotobiotic pig serum samples from Virginia and commercial OIE disease-free porcine serum as the positive-control reference serum (pos). (B) Representative results of TTSuV1a WB analyses of conventional pig sera from a farm in Wisconsin. Purified 1a-ORF1 protein was used as the antigen. Sera that tested negative for both TTSuV1a and TTSuV1b antibodies by WB were pooled and used as the negative-control reference serum. Open arrowheads indicate the truncated ORF1 protein of the expected size. Only the bands in green were considered positive. M, protein markers. (C) TTSuV1a or TTSuV1b ELISA results for the seven Virginia gnotobiotic pig serum samples and positive- and negative-control reference sera.