Table 2.
Reactivities of anti-TTSuV1a, anti-TTSuV1b, anti-TTSuV2, prebleed rabbit, and anti-human TTV (AK47) sera in PCV1-free PK-15 cells transfected with plasmids encoding truncated ORF1 proteins from TTSuV1a, TTSuV1b, and TTSuV2, respectively, as determined by IFA
| Antibody transfection | Antibody reactivitya |
||||
|---|---|---|---|---|---|
| Anti-TTSuV1a | Anti-TTSuV1b | Anti-TTSuV2 | Prebleed rabbit serum | Anti-human TTV (AK47) | |
| pTri-1aORF1 | ++ | + | − | − | − |
| pTri-1bORF1 | + | ++ | − | − | − |
| pTri-2cORF1 | − | − | ++ | − | − |
| Mock | − | − | − | − | − |
a
The intensity of the fluorescent signal was determined visually and is expressed as ranging from − to ++.