Fig 4.

Virus particle production with L4-22K mutant viruses. (A) CsCl equilibrium density gradient profiles of virus particles produced from Ad5-WT-, Δ22K-, and 22K⁻-infected A549 and N52.E6-Cre cells and Δ22K-infected TetC4-IVa2 and TetC4-33K cells. Arrows and arrowheads indicate mature virions (MV) and empty capsids (EC), respectively. (B) The results of silver staining of protein components of MV and EC isolated from the virus particles indicated in panel A are shown; in addition, MV and EC isolated from Ad5-Ψ-loxP-infected N52.E6 (MV) and N52.E6-Cre (EC) cells are shown. Protein designations are indicated on the left. M, molecular weight markers. (C) Western blot analysis of protein components of MV and EC isolated from the virus particles indicated in panel B. Protein designations are indicated on the right.