Fig 3.

LGP2 inhibits STAT1 activation in human cells infected with the H3N2 Ud virus. (A) HeLa cells were transfected with plasmids expressing LGP2 or the LGP2 RD or with an empty plasmid for 24 h, followed by infection with the H3N2 Ud virus. (B) Levels of phosphorylated STAT1, phosphorylated IRF3, and V5-tagged LGP2 and LGP2 RD were assayed by immunoblots. (C) Increase in IFN-β pre-mRNA and IFN-β mRNA after 6 h of infection of HeLa cells with the H3N2 Ud virus, as measured by quantitative RT-PCR carried out in triplicate.