Fig 8.

YY/AA and YY/FF mutations of the β1 CTD alter talin and kindlin binding and activation of integrin β1. (A) The binding affinities of β1 integrin with cytoplasmic proteins talin and kindlin were determined using the SILAC assay performed with WT, YY/AA, YY/FF, and scrambled (scr) β1 integrin peptides. (B) The activation of the β1 integrin was determined by quantifying the amount of antibody 12G10 that bound to the active conformation of human β1 integrin using a FACS assay. The percentage of binding was in each case normalized with the total surface expression of integrin and expressed as an activation index. Total surface expression was quantified by using the AIIB2 antibody in the FACS assay. The error bars indicate SD.