Table 1.

Unpublished primers and cycling conditions

Gene and primer	Sequence	Product size (bp)	Nested PCR cycling conditions
SSU-rRNA
RPLU5New	5′-CYTGTTGTTGCC TTAAAC TTC-3′	1,200	95°C for 5 min; 25 cycles of 58°C for 2 min, 72°C for 2 min, and 94°C for 1 min; 58°C for 2 min; 72°C for 5 min
pfcrt, nested
Nest 2 forward D1	5′-TGTGCTCATGTGTTTAAACTT-3′	145	94°C for 30 s, 30 cycles of 56°C for 30 s, and 65°C for 1 min; 65°C for 5 min; 15°C for 5 min
Nest 2 reverse D2 (12)	5′-CAAAACTATAGTTACCAATTTTG-3′
pfmdr1, fragment 3, seminested
Nest 1 forward MDRFR3N1	5′-GCATTTTATAATATGCATACTG-3′	234	94°C for 1 min; 30 cycles of 94°C for 30 s, 55°C for 30 s, and 68°C for 45 s; 68°C for 2 min; 15°C for 2 min
Nest 1 reverse MDRFR3R1	5′-GGATTTCATAAAGTCATCAAC-3′
Nest 2 forward MDRFR3N2	5′-GGTTTAGAAGATTATTTCTGTAA-3′	201
Nest 2 reverse MDRFR3R1	5′-GGATTTCATAAAGTCATCAAC-3′
pfmdr1, fragment 4, seminested
Nest 1 forward MDRFR4N1	5′-CAAACCAATCTGGATCTGCAGAAG-3′	194
Nest 1 reverse MDRFR4R1	5′-CAATGTTGCATCTTCTCTTCC-3′
Nest 2 forward MDRFR4N2	5′-GATCTGCAGAAGATTATACTG-3′	182
Nest 2 reverse MDRFR4R1	5′-CAATGTTGCATCTTCTCTTCC-3′