Fig 5.

Intracellular oxidative stress during the short-time treatment with vitamin B₁₂. Epimastigotes were treated with B₁₂ (15, 30, or 60 μM) for 3, 7, or 24 h. Intracellular oxidative stress then was evaluated by flow cytometry (n = 20,000 cells/analysis). (a) Histograms corresponding to untreated cells (curve 1, control) and treated with 30 μM B₁₂ for 3, 7, or 24 h (curves 2, 3, and 4, respectively). (b) Time course of the Gm_t/Gm_c ratio (see Materials and Methods) for parasites treated with the different concentrations of B₁₂.