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. 2012 Oct;56(10):5096–5102. doi: 10.1128/AAC.00732-12

Fig 3.

Fig 3

(A) Southern blot hybridization with the p1-9 probe of XbaI-digested genomic DNAs of S. enterica serovar Typhimurium DT104 control strain BN9181 carrying SGI1 (lane 2), serovar Virchow control strain SV20 carrying SGI1-J4 (lane 3), serovar Kentucky strain 10-90 (lane 4), serovar Kentucky strain 93-6429 (lane 5), serovar Virchow control strain B98 carrying SGI1-J3 (lane 6), serovar Kentucky strain 19-85 (lane 7), serovar Kentucky strain 1-86 (lane 8), serovar Kentucky strain 12-89 (lane 9), and serovar Kentucky strain 92-6276 (lane 10). Kbp DNA marker (Smartladder, Eurogentec, Seraing, Belgium) (lane 1). (B) Genetic organization of the complex class 1 integron in SGI1-J4 and SGI1-J6 from S. Kentucky strains. Black and gray arrows correspond to SGI1 antibiotic resistance genes and chromosomal genes flanking SGI1, respectively. IS elements are indicated by boxes containing black hatched arrows representing the transposase genes. DR-L and DR-R are the 18-bp left and right direct repeats, respectively, bracketing SGI1. IRi and IRt are 25-bp imperfect inverted repeats defining the left and right ends of complex class 1 integrons. The usual insertion point of the complex integron In104 or variants of it in SGI1 and the 5-bp target site duplication flanking the complex integron (InSGI1-J4 and -J6) in ORF S023 are indicated. The p1-9 probe and XbaI restriction sites (X) are indicated. PCRs carried out to map the InSGI1-J complex integrons are indicated by light-gray arrows under each map.