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Changes in membrane potential and cell viability decrease in parallel for both daptomycin and CB-183,315. Changes in S. aureus membrane potential (as measured by the ratio of fluorescence emission intensities at 610 and 530 nm) and cell viability (from samples serially diluted and plated on TSAB) were plotted over time following incubation with 2.5 μg/ml daptomycin or 1.25 μg/ml CB-183,315 (both at 5× the MIC). Values are expressed as percentages of the untreated control cells.
