Fig 6.

PlyG-mediated spore binding and killing. (A) Lysin activity of PlyG on B. anthracis vegetative cells. One unit of two-step-purified PlyG (open squares) displays high activity against B. anthracis cells, in contrast to the buffer control (solid squares). (B) Effect of PlyG on B. anthracis spores. Heat-activated B. anthracis spores were treated with PlyG (10 U) in the presence of either 100 mM l-alanine (light gray) or d-alanine (white) for different times. The resulted CFU were counted and are contrasted with results for the blank control (gray). EG1 can bind to inactivated (C) or live (D) B. anthracis spores in the presence of either l-alanine or d-alanine, and the relative fluorescence intensity is not obviously different from that of the buffer control.