Table 2.
Effects of SPase expression levels on the arylomycin A-C16 sensitivity of E. coli
| aTc concn (ng/ml) | Arylomycin A-C16 MIC (mg/ml) for straina: |
||||||
|---|---|---|---|---|---|---|---|
| MG1655 (NEV/Wt) | PAS0162 (Wt/NCS) | PAS0260 (NEV/P84L) | PAS0275 (P84L/P84L) | PAS0232 (NEV/P84S) | PAS0234 (P84S/P84S) | PAS0216 (P84S/NCS) | |
| 0 | >64 | >64 | 1 | 2 | 8 | 16 | 1 |
| 4 | >64 | >64 | 1 | 2 | 8 | >64 | 2 |
| 8 | >64 | >64 | 1 | 4 | 8 | >64 | 4 |
| 16 | >64 | >64 | 1 | 8 | 8 | >64 | 64 |
| 32 | >64 | >64 | 1 | 16 | 8 | >64 | >64 |
| 64 | >64 | >64 | 1 | 16 | 8 | >64 | >64 |
| 128 | >64 | >64 | 1 | 16 | 8 | >64 | >64 |
a
For each strain, ectopic/chromosomal SPases are in parentheses. Wt, wild-type SPase; P84L and P84S, arylomycin-sensitive mutants (see the text); NEV, no SPase expression vector; NCS, no chromosomal SPase.