Fig 2.
Schematic diagram showing the genomic organization of the novel SCCmec-SCC composite island (CI) SCCmec IIE-SCCM1 identified in the ST8-MRSA spa type t190 isolate AR13.1/3330.2 (GenBank accession number HE858191) (a), the SCCM1-SCCarc-SCCmec element previously reported in ST8-MRSA spa type t024 isolate M1 (HM030720) (b), the ACME- and SCC-CIs previously reported in S. epidermidis ATCC 12228 (NC004461) (c), and SCCmec VIII previously described in ST8-MRSA spa type t008 isolate C10682 (FJ390057) (d). The structure of the novel SCCmec IIE-SCCM1 element was determined by high-throughput whole-genome sequencing of AR13.1/3330.2 and was confirmed using primers spanning the SCCmec/SCC region. The areas surrounded by the red square indicate the regions of high similarity between SCCM1 identified in isolate AR13.1/3330.2 and the other SCCmec/SCC elements.