Table 1.

IC₅₀ calculation after 48-h period of exposure to selected compounds of L. infantum promastigotes, ex vivo-infected splenocytes, and uninfected splenocytes^a

Drug (reference)	IC50 forb:			SI48hc
	L. infantum promastigotes	Amastigotes	Uninfected splenocyte culture
Paromomycin	42.41 ± 1.65 μM	9.20 ± 0.01 μM	15.70 ± 3.54 mM	1,706.5
Camptothecin (22)	1.12 ± 0.13 μM	0.03 ± 0.01 μM	0.62 ± 0.13 μM	20.7
Indotecan	0.10 ± 0.08 μM	0.10 ± 0.05 μM	57.16 ± 6.01 μM	571.6
AM13-55	1.02 ± 0.09 μM	0.10 ± 0.37 μM	48.37 ± 3.68 μM	483.7

^aIC₅₀ at 48 h of the compounds selected in freshly uninfected splenocyte culture and SI_48h values between this cell line and infected splenocytes with L. infantum amastigotes ex vivo were calculated from the dose-response curves determined in triplicate in separate experiments after performing a nonlinear fitting with the SigmaPlot software program.

^bMean ± SD.

^cSelectivity index; SI_48h, IC₅₀ for amastigotes/IC₅₀ for infected splenocytes.