Table 2.

Sequences of the primers and the PCR programs used to identify S. suis serotypes 3, 4, 5, 8, 10, 19, 23, and 25

Serotype	Primer	Sequence (5′→3′)	PCR program	Amplicon length (bp)
3	cps3L-P1	AGTTGGTATTTGTTTAGG	5 min at 94°C and 30 amplification cycles (94°C at 30 s, 30 s at 50°C, and 30 s at 72°C)	340
	cps3L-P2	GGAAGATTTAGCAGTTTT
4	cps4L-P1	TACTTCAGTTAGGTTCCTCGCA	5 min at 94°C and 30 cycles of 30 s at 94°C, 30 s at 50°C, and 30 s at 72°C	690
	cps4L-P2	CTCTCCACTTATTCACTTTCCC
5	cps5I-P1	TTTTCGTTGTATTTTCCAAA	5 min at 94°C and 30 cycles of 30 s at 94°C, 30 s at 50°C, and 30 s at 72°C	262
	cps5I-P2	TCCAAACATTATCCCCTATT
8	cps8K-P1	ATCTGTTGTAATGGTCGC	5 min at 94°C and 30 cycles of 30 s at 94°C, 30 s at 50°C, and 1 min at 72°C	1,200
	cps8K-P2	CTATTCCTCTTGGTGTGA
10	cps10M-P1	TCACTACCACGGAATGCT	5 min at 94°C and 30 cycles of 30 s at 94°C, 30 s at 50°C, and 1 min at 72°C	898
	cps10M-P2	CAAAGGTCACCCCAGAAT
19	cps19N-P1	AGGCTACAAAGCGATAGAACT	5 min at 94°C and 30 cycles of 30 s at 94°C, 30 s at 50°C, and 30 s at 72°C	391
	cps19N-P2	ACCAAAGAATCAACCAATAAA
23	cps23J-P1	GCTATCAGGAATACACAAAG	5 min at 94°C and 30 cycles of 30 s at 94°C, 30 s at 50°C, and 1 min at 72°C	758
	cps23J-P2	AGTGGAATAACAACAAAAAT
25	cps25G-P1	GATTTTTGTGTGACTGTGGG	5 min at 94°C and 30 cycles of 30 s at 94°C, 30 s at 50°C, and 30 s at 72°C	478
	cps25G-P2	TTCGGTAACTTCTCGTGT