Table 2.

Reagent costs and labor time between the standard protocol and MALDI protocol

Species or species group	No. of isolates per year	Standard protocol		MALDI protocol
		Reagent cost ($)	Labor time (h:min)	Reagent cost ($)	Labor time (h:min)
Staphylococcus aureus	7,059	15,747.53	76:38	2,523.63	93:21
Staphylococcus lugdunensis	65	180.99	1:44	48.11	2:58
Staphylococcus saprophyticus	63	243.81	1:03	21.87	0:47
Streptococcus anginosus group	418	3,824.28	53:05	240.17	11:19
Streptococcus pyogenes	1,419	3,683.99	55:48	492.55	17:44
Streptococcus agalactiae	3,516	10,866.87	199:06	1,292.22	46:32
Streptococcus dysgalactiae	376	1,278.06	24:01	142.38	5:07
Streptococcus pneumoniae	338	1,095.08	27:13	786.29	32:23
Viridans streptococcus group	699	7,099.89	90:48	613.70	40:13
Enterococcus faecium	909	3,718.73	21:00	347.07	12:29
Enterococcus faecalis	3,346	10,630.48	32:21	1,254.34	45:10
Enterococcus raffinosus	72	791.30	10:12	202.32	6:22
Enterococcus species	76	839.60	10:13	163.90	3:29
Micrococcus luteus	54	102.02	0:42	39.97	2:28
Citrobacter freundii group	263	524.64	3:24	91.29	3:17
Citrobacter koseri	260	510.64	3:20	90.25	3:15
Citrobacter sp.	58	110.75	2:05	20.13	0:43
Enterobacter aerogenes	381	715.46	4:23	132.25	4:45
Enterobacter cloacae group	883	1,921.35	10:55	306.50	11:02
Enterobacter sp.	51	428.95	5:42	35.41	1:16
Escherichia coli	8,816	17,445.65	109:53	3,158.82	113:45
Klebsiella oxytoca	322	676.80	3:34	111.77	4:01
Klebsiella pneumoniae	2,884	5,598.89	32:33	1,090.44	39:16
Morganella morganii	201	611.22	3:49	69.77	2:30
Proteus mirabilis	1,315	5,050.28	48:01	456.45	16:26
Proteus vulgaris/penneri	57	122.20	0:39	23.74	0:51
Providencia stuartii	82	151.52	0:54	28.46	1:01
Salmonella enteritidis	118	218.05	1:18	40.96	1:28
Acinetobacter baumannii group	284	1,185.44	3:39	107.54	3:52
Achromobacter sp.	190	2,460.26	2:57	190.10	10:29
Acinetobacter sp.	65	968.87	1:37	22.56	0:48
Burkholderia cepacia	85	8,187.99	1:39	7,195.85	1:03
Pseudomonas aeruginosa	4,974	22,792.85	70:15	2,039.79	82:39
Pseudomonas fluorescens/putida	61	801.10	0:58	21.17	0:45
Serratia marcescens	374	783.35	4:19	129.82	4:40
Stenotrophomonas maltophilia	732	3,187.41	12:50	293.17	10:33
Campylobacter jejuni/coli	57	267.34	4:42	19.79	0:42
Haemophilus influenzae	404	739.41	12:30	140.23	5:03
Haemophilus species, but not H. influenzae	95	110.27	1:03	32.98	1:11
Moraxella catarrhalis	120	317.10	2:40	41.65	1:30
Actinomyces sp.	59	5,623.30	0:09	77.10	5:02
Corynebacterium striatum	201	1,568.12	0:39	83.72	3:00
Propionibacterium acnes	184	446.99	4:09	63.87	2:18
Bacteroides fragilis	465	4,068.84	36:04	161.41	5:48
Clostridium species, but not C. perfringens	78	3,439.08	9:27	47.52	2:42
Peptostreptococcus sp.	154	608.17	14:48	401.44	15:09
Prevotella sp.	254	1,699.49	17:15	585.00	12:31
Candida albicans	1,675	1,045.88	22:47	1,401.85	118:52
Candida glabrata	933	890.81	64:52	787.86	66:48
Candida krusei/inconspicua	147	365.74	10:52	115.57	9:48
Candida lusitaniae	59	27.14	3:26	46.39	3:56
Candida parapsilosis	247	471.03	20:08	224.07	19:00
Candida tropicalis	294	2,029.46	25:31	295.35	25:02
Cryptococcus neoformans	74	107.97	6:34	130.16	9:19
Saccharomyces cerevisiae	51	262.87	6:43	40.10	3:24
“Uncommon” speciesa	1,428	–b	–	1,093.03	69:14
All speciesc	47,845	158,645.33	1,197:24	29,613.84	1,019:28

^aSpecies where less than 50 isolates are encountered per year.

^bThese values are not required to estimate annual savings, as they are cancelled out in the cost differences.

^cThe total values for all species are shown in boldface type.