Table 1.

Real-time PCR testing of formalin-fixed paraffin-embedded tissues where the histological report suggested histoplasmosis without culture confirmation

Code	Specimen source	Histopathology reporta	PCR resultb
1	Lung	Numerous yeast forms compatible with Histoplasma	+
2	Lung	Numerous budding yeast forms compatible with Histoplasma species	+
3	Lung	Budding yeast forms morphologically consistent with Histoplasma capsulatum	+
4	Lung	Fungal organisms morphologically consistent with histoplasmosis identified	+
5	Lung	Necrotizing granulomatous inflammation with associated abscess formation suggesting Histoplasma	+
6	Lung	Caseating granulomatous inflammation with numerous fungal yeast forms morphologically consistent with Histoplasma species	+
7	Lung	Fungal yeast forms consistent with Histoplasma	+
8	Lung	Necrotizing granulomatous inflammation consistent with histoplasmosis	+
9	Lung	Necrotizing granulomatous inflammation with abundant fungal organisms consistent with Histoplasma species	+
10	Lung	Small budding yeasts morphologically consistent with Histoplasma capsulatum	−
11	Lung	Small budding fungal yeast forms morphologically consistent with Histoplasma capsulatum	−
12	Lung	Focal necrotizing granuloma formation containing fungal organisms consistent with histoplasmosis	−
13	Lung	Necrotizing granuloma with small budding yeast forms suggestive of Histoplasma	−
14	Lung	Rare yeast forms compatible with Histoplasma species	−
15	Lung	Bilateral multiple calcified granulomata with yeast forms consistent with histoplasmosis	−
16	Lymph node	Rare yeast-shaped fungal organisms suggesting Histoplasma	+
17	Lymph node	Necrotizing granuloma containing budding yeast consistent with Histoplasma	+
18	Lymph node	Yeast forms morphologically consistent with Histoplasma	−
19	Lymph node	Caseating granulomas, rare yeast morphologically suggestive of Histoplasma	−
20	Spleen	Foci of hylanized centrally necrotic granulomatous inflammation with small yeast forms consistent with Histoplasma capsulatum	−
21	Liver	Necrotizing granuloma with small budding yeast forms morphologically consistent with Histoplasma capsulatum	−

^aUsing Gomori's methenamine silver stain method.

^b+, positive; −, negative.