Table 1.
Oligonucleotide primers used for T. vaginalis MLST
| Locus (abbreviation) | Primer |
Size (bp) |
||
|---|---|---|---|---|
| Orientation | Sequences (5′–3′) | Gene | Amplicon | |
| Tryptophanase (P1)a | Forward | CGTCAACATCGGTGGCTTCA | 1,451 | 489 |
| Reverse | GCGACAGCGACGACATTCAT | |||
| Glutaminase (P3) | Forward | GTGCCATTACAACAGCATCG | 886 | 451 |
| Reverse | CCAAGTATAGCTCCGCTGAC | |||
| Family T2 aparaginase-like threonine peptidase (P6) | Forward | GAACAGGAGCACCAGCAGAA | 990 | 412 |
| Reverse | TCTCTAGCAACGCAGCCAAC | |||
| Alanyl tRNA synthetase (P8) | Forward | TCTGTCCAGGATGGTGTCTT | 3,075 | 494 |
| Reverse | ACGCCTTCCTCCTTCATCTT | |||
| DNA mismatch repair protein (P13)b | Forward | TCATCGGCCAATGGAACCAA | 1,758 | 491 |
| Reverse | TCCGTGCGGACAATTCCAAG | |||
| Serine hydroxymethyltransferase (P14) | Forward | GCTGAGTGACGGTGGACATT | 1,356 | 449 |
| Reverse | GAAGATGAGGTCCTCCTTGA | |||
| Mannose 6-phosphate isomerase (P16) | Forward | AGCCAGTTGGCTTCTGAGTT | 1,128 | 459 |
| Reverse | AACAATTCCGCAAGCTGGAG | |||