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. 2012 Oct;50(10):3293–3300. doi: 10.1128/JCM.00643-12

Table 1.

Oligonucleotide primers used for T. vaginalis MLST

Locus (abbreviation) Primer
Size (bp)
Orientation Sequences (5′–3′) Gene Amplicon
Tryptophanase (P1)a Forward CGTCAACATCGGTGGCTTCA 1,451 489
Reverse GCGACAGCGACGACATTCAT
Glutaminase (P3) Forward GTGCCATTACAACAGCATCG 886 451
Reverse CCAAGTATAGCTCCGCTGAC
Family T2 aparaginase-like threonine peptidase (P6) Forward GAACAGGAGCACCAGCAGAA 990 412
Reverse TCTCTAGCAACGCAGCCAAC
Alanyl tRNA synthetase (P8) Forward TCTGTCCAGGATGGTGTCTT 3,075 494
Reverse ACGCCTTCCTCCTTCATCTT
DNA mismatch repair protein (P13)b Forward TCATCGGCCAATGGAACCAA 1,758 491
Reverse TCCGTGCGGACAATTCCAAG
Serine hydroxymethyltransferase (P14) Forward GCTGAGTGACGGTGGACATT 1,356 449
Reverse GAAGATGAGGTCCTCCTTGA
Mannose 6-phosphate isomerase (P16) Forward AGCCAGTTGGCTTCTGAGTT 1,128 459
Reverse AACAATTCCGCAAGCTGGAG
a

Determined to have likely resulted from lateral genetransfer (10).

b

Single-copy gene used for population genetics analysis of T. vaginalis by Conrad, et al. (12, 13).