Fig 4.
Expression of the genes for Lysobacter sp. strain XL1 lytic endopeptidases AlpA and AlpB in E. coli. A lytic-activity test is shown. Cells of E. coli BL21(DE3) transformed with vector pET-29a (sector 3) or with plasmid pALPI-29a (sector 2) or pALPII-29 (sector 1) bearing the alpA or alpB gene, respectively, were grown on an agarized medium containing cell walls of S. aureus 209-P (see Materials and Methods). Lytic activity is seen as clarification zones around colonies.