Fig 4.

Minimum spanning tree of 16 MLV-HRMA genotypes obtained from 46 isolates of Geobacillus spp. Each node in a tree represents a different MHT, and the genotype number is indicated by the number in the node itself. Clonal groups are encased in a thin black line. Single-locus-variant genotypes are linked by a solid black line, while double- and triple-locus-variant genotypes are linked by solid gray and dashed lines, respectively. Node size represents the number of isolates associated with each genotype. Black shading indicates powders with high spore counts (>10⁴ CFU ml⁻¹), dark gray shading indicates powders with medium spore counts (10² to 10⁴ CFU ml⁻¹), and light gray shading indicates powders with low spore counts (<10² CFU ml⁻¹).