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Pigment production by wild-type P. stewartii (DC283) and the crtB∷Mar2xT7 (crtB) mutant. (A) Single colonies of the crtB transposon mutant obtained were nonpigmented on nutrient agar. (B) Bacterial pellets accumulated a yellow carotenoid pigment in the wild-type strain DC283 but not the crtB mutant. Pigmentation was restored when a wild-type copy of crtB was supplied in trans on plasmid pMOJ14 in strain crtB∷Mar2xT7/pMOJ14 (crtB/crtB+).
