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Spectral analysis of the P. stewartii carotenoid pigment. The methanol-extractable pigment from wild-type P. stewartii (DC283) has an absorption spectrum with a peak at 451 nm and a shoulder at 475 nm and with the maximum absorbance (λmax) at 450 nm, which was similar to that of the β-carotene standard. The crtB∷Mar2xT7 (crtB) mutant did not have detectable pigment in the methanol extract.
