Tolerance to UV radiation. Wild-type P. stewartii (DC283), the crtB∷Mar2xT7 (crtB) mutant, and the crtB∷Mar2xT7/pMOJ14 (crtB/crtB+) complemented strain were grown to stationary phase in LB medium, diluted in 1× PBS, and exposed to near-UV-A irradiation (320 to 400 nm) for 30 s. CFU were enumerated, and the percent growth inhibition was normalized to that for the untreated control. The crtB∷Mar2xT7 (crtB) mutant was significantly more sensitive to UV radiation than the WT (DC283) strain, and UV tolerance was fully restored in the crtB∷Mar2xT7/pMOJ14 (crtB/crtB+) complemented strain.