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The EsaI/EsaR quorum-sensing system regulates carotenoid pigment production. Wild-type P. stewartii (DC283) and the ΔesaI quorum-sensing signal synthase mutant, with or without exogenous 3-oxo-C6-AHL (AHL) signal, were grown in LB medium for 48 h. Pigment extraction and quantification were performed as described in Materials and Methods. The ΔesaI mutant produces less pigment, and pigment production is restored when the AHL signal is added to the medium.
