Table 2.
Primers used in this study
| Primer type and name | Primer sequence (5′→3′)a | Reference |
|---|---|---|
| ITS1 | TCCGTAGGTGAACCTGCGG | 52 |
| ITS4 | TCCTCCGCTTATTGATATGC | 52 |
| Degenerate primers | ||
| AXP1 forward | GAYACNGGNTCNTCNGAY | This study |
| AXP1 reverse | NGANGTNGCNGARTCNAR | This study |
| Inverse primers | ||
| Mpulch_ IPCR_ F1 | CAGATCTCGGCAAGGGCTCGTTGAT | This study |
| Mpulch_ IPCR_ R1 | TGCGCAAACTGGAAATTGGAAAGCA | This study |
| Mpulch_ IPCR_ F2 nested | GGTGAACGCCCCAGTTCT | This study |
| Mpulch_ IPCR_ R2 nested | AGCTGGAACTGTCTGGGCTA | This study |
| Capi_IPCR_F1 | CAGTGCCCAGAATGGAAGCAGTGTG | This study |
| Capi_IPCR_R1 | ACACCGGCAGTTTCGCCCTGAGTAG | This study |
| Capi_IPCR_F2 nested | TGGTAACAAGTCGAATGGTGTG | This study |
| Capi_IPCR_R2 nested | CCTTGAAGTTTGTCTGATTTGTG | This study |
| Primers for full gene | ||
| MpAPr1-F | GGATCCATGCAATTCCTCACTCTTCTTTC | This study |
| MpAPr1-R | CTCGAGTTAAGCACTTATGATGTTTGACGA | This study |
| CaAPr1-F | GGATCCATGGTACTAGCTAAGAACTATGTT CATTTA | This study |
| CaAPr1-R | CTCGAGTTAGTTGACAGATCCGGGAAT | This study |
| 5′-KPNPGK-631 | GGGGTACCCTTTATTTTGGCTTCACCC | 48 |
| NL4 | GGTCCGTGTTTCAAGACGG | 23 |
| NL1 | GCATATCAATAAGCGGAGGAAAAG | 23 |
| MpAPr1-qPCR-fw | ACACCCAAGGCGTCATACTC | This study |
| MpAPr1-qPCR-rev | ACAGGTCAATCGGGTACAGC | This study |
| ACT1-qPCR-fw | CTCCATGCCTCACGGTATTT | This study |
| ACT1-qPCR-rev | CTCCTGCTCAAAGTCCAAGG | This study |
a
Underlined sequences indicate restriction sites: GGATCC, BamHI; CTCGAG, XhoI; GGTACC, KpnI.