Table 3.
PCR amplification programs
| Primer pair | Initial denaturation temp (°C)/time (min) | Main cycling conditions |
Final extension temp (°C)/time (min) | |||
|---|---|---|---|---|---|---|
| No. of cycles | Temp (°C)/time (s) |
|||||
| Denaturing | Annealing | Extension | ||||
| ITS1/ITS4 | 95/5 | 40 | 95/1 | 58/1 | 72/1 | 72/7 |
| AXP1 forward/reverse | 94/7 | 45 | 94/30 | 50/60 | 72/0.6 | 72/7 |
| Mpulch_ IPCR_ F1/R1 | 94/2 | 30 | 94/20 | 68/20 | 68/5 | 68/5 |
| Capi_IPCR_F1/R1 | 94/2 | 30 | 94/20 | 60/20 | 68/5 | 68/5 |
| Mpulch_ IPCR_ F2/R2 nested | 94/2 | 30 | 94/20 | None | 68/5 | 68/5 |
| Capi_IPCR_F2/R2 nested | 94/2 | 30 | 94/20 | None | 68/5 | 68/5 |
| MpAPr1-F/R | 98/0.5 | 35 | 98/10 | 58/20 | 72/0.5 | 72/7 |
| CaAPr1-F/R | 98/0.5 | 35 | 98/10 | 58/20 | 72/0.5 | 72/7 |
| 5′-KPNPGK-631/Mpulch_ IPCR_ R2 | 96/2 | 35 | 94/30 | 58/30 | 72/0.6 | 72/10 |
| NL4/NL1 | 98/0.5 | 35 | 98/10 | 58/20 | 72/0.5 | 72/7 |