Fig 2.
Maps of the FTRp-luxCDABE vectors. (A) The inducible vector, pEDL40, is a stable Hygr E. coli-F. tularensis shuttle vector that contains the hybrid Francisella FTR promoter driving the luminescence operon, luxCDABE. The tetR gene, constitutively expressed from the Francisella rpsL promoter, is divergently transcribed. (B) The vector pEDL41 is pEDL40 with the tetR gene deleted. This allows the constitutive expression of the lux operon from FTRp. (C) The repressible vector, pEDL47, is a Hygr, stable E. coli-F. tularensis shuttle vector that contains FTRp-luxCDABE. The TetR r1.7 gene (a revTetR), constitutively expressed from the Francisella rpsL promoter, is divergently transcribed.