Fig 3.

Results of ChIP-PCR assay with or without His tag monoclonal antibody. DNA was amplified by primers designed against the regulatory regions. Lanes 1 and 2 represent the positive controls using chromatin as the template for PCR. The sizes of the resulting fragments are 438 bp (using primers pipF1 and pipR2; Table 1) and 152 bp (using primers pipF1 and pipR1; Table 1), respectively. Negative controls with no antibody are presented in lanes 3 and 4. The results with the addition of anti-His antibody are shown in lanes 5 and 6. The no-template negative control is not shown.