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XccR interacts with HrpX in vitro. The HrpX-his, MBP, and XccR-MBP strains were used in a His pulldown assay. The precipitated proteins were detected by Western blotting using anti-XccR and anti-MBP antibodies. The recombinant HrpX did not precipitate with MBP (lane 1) but did with XccR-MBP (lane 2). In lane 3, MBP was used as a positive control for immunoblot detection by an anti-MBP antibody. Lane 4 shows an immunoblot of 2 μg purified XccR-MBP.
