Table 2.

Legionella clones and distribution of operational taxonomic units before and 72 h after the two heat shock treatments and the second chemical treatment

Test and time point	Operational taxonomic unit				Organism with best-matching sequence
	Name	No. of clones	% Similarity	Affiliation	Accession no.	Name
First heat shock treatment
Before treatment	T0_HS1_g.02	1	96.8	Legionella drancourtii	X97366.2	Legionella drancourtii
	T0_HS1_f.02	12	99.84	Legionella anisa	AY744776.1	Legionella anisa
	T0_HS1_f.01	6	99.08	Legionella pneumophila subsp. pneumophila	AE017354.1	Legionella pneumophila subsp. pneumophila
	T0_HS1_e.01	8	100	Legionella taurinensis	DQ667196.1	Legionella taurinensis
Total no.	4	27
72 h after treatment	72h_HS1_e.03	30	99.69	Legionella taurinensis	DQ667196.1	Legionella taurinensis
Total no.	1	30
Second heat shock treatment
Before treatment	T0_HS2_14	23	99.84	Legionella pneumophila	EU054324.1	Legionella pneumophila
	T0_HS2_12	7	99.08	Legionella taurinensis	DQ667196.1	Legionella taurinensis
Total no.	2	30
72 h after treatment	72h_HS2_26	16	99.84	Legionella taurinensis	DQ667196.1	Legionella taurinensis
	72h_HS2_25	16	99.84	Legionella pneumophila subsp. pneumophila	AE017354.1	Legionella pneumophila subsp. pneumophila
Total no.	2	32
Second chemical treatment
Before treatment	T0_C2_h.04	2	97.11	–	EU557015.1	Uncultured bacterium
	T0_C2_f.04	2	100	Legionella pneumophila subsp. pneumophila	AE017354.1	Legionella pneumophila subsp. pneumophila
	T0_C2_d.02	11	99.84	Legionella anisa	AY744776.1	Legionella anisa
	T0_C2_d.01	10	99.84	Legionella pneumophila	EU054324.1	Legionella pneumophila
Total no.	4	26
72 h after treatment	72h_C2_e04	19	99.84	Legionella anisa	AY744776.1	Legionella anisa
Total no.	1	19