Fig 5.

Results of HPLC analyses of intracellular sugar content during early germination of P. rubens incubated in MEB (open symbols connected by a solid line) or in DW (solid symbols connected by a dashed line) at 30°C. The change in the sugar-to-mannitol ratio measured at t = 0 was used as an indicator of metabolic activity. The amount of mannitol at t = 0 was taken to normalize the data because this was the principal sugar present in dormant cells. A change in the intracellular sugar content ratio was observed within 30 min of incubation in MEB, whereas no alterations were observed in conidia after 210 min of incubation in DW.