Fig 2.
ComGC stabilization by DsbA. S. aureus strains were grown for 7 h in LB broth as described in Fig. 1 in the presence or absence of β-mercaptoethanol (final concentration, 1 or 2.5 mM). The preparation of cell extracts, SDS-PAGE, and Western blotting with specific antibodies against ComGC or σH were performed as described in the legend to Fig. 1. The chromosomal dsbA gene was deleted from S. aureus strain RN4220 as previously described (2, 14). Primers used for strain construction are listed in Table 1. Lanes relating to strains that carry pRIT:sigH for σH production are indicated by “H”; lanes relating to control strains with the empty vector pRIT5H that do not produce σH are indicated by “v.”