Table 1.
Primers used in this study
| Primer name and purpose | Sequence (5′→3′)a |
|---|---|
| Construction of S. aureus comC mutant | |
| ComC-F1 | CGAGATGGTCAAACATTTAAG |
| ComC-R1 | TCACGTCAGTCAGTCACCATGGCAATGACAACCTCCTTATGTAAA |
| ComC-F2 | TGCCATGGTGACTGACTGACGTGAAAATTAAAGAAATGGTAA |
| ComC-R2 | AACTGCGATGATTGCATTGGC |
| Construction of S. aureus comGC mutant | |
| ComGC-F1 | GCTCAATAAGATAAACTTTGT |
| ComGC-R1 | CTACGTCAGTCAGTCACCATGGCAATATTAACCTCCATTATTTTA |
| ComGC-F2 | TGCCATGGTGACTGACTGACGTAGAAAGCAGTCAGCATTTAC |
| ComGC-R2 | GATTCATCATTGGTATCAATA |
| Construction of S. aureus dsbA mutant | |
| DsbA-F1 | ATTTCTTTGGATATTTATATT |
| DsbA-R1 | CTACGTCAGTCAGTCACCATGGCAAATAACTCCTATTCATAT |
| DsbA-F2 | TGCCATGGTGACTGACTGACGTAGTCTTAATTGTTGAGATCA |
| DsbA-R2 | CTTTCGTTATAGTTTTCCCAC |
| Construction of pCN51:comC for S. aureus comC overexpression | |
| ComC-F | CAGCCGGATCCCATAAGGAGGTTGTCATTTGGTAG (BamHI) |
| ComC-R | CGGAATTCCTTTAATTTTCAAAAATATACGCCTCC (EcoRI) |
a
Overlapping parts are shown in boldface. Restriction sites used for cloning are underlined (with the restriction endonuclease given in parentheses.