Fig 6.

atf3 mutants mount a starvation-like transcriptional program. Total RNAs from fed and fasted (8 h) atf3⁷⁶/Y and yw/Y control larvae were subjected to qRT-PCR. Expression of lip3, thor, relish, and atf3 itself was upregulated in fed atf3 mutants relative to the control, and starvation further induced the mRNAs in all larvae. A luciferase assay in larval lysates showed activation of a drosomycin (drs::luc) transgenic reporter in fed atf3 mutant larvae, and the reporter activity was further stimulated by starvation for 14 h. The data are means and SEM; n ≥ 4; *, P < 0.05; **, P < 0.01; and ***, P < 0.001.