Table 1.
Identities of cloned shRNAs
| Gene namea | Gene product | No. of shRNAsb |
|---|---|---|
| ZNF193 | Zinc finger protein 193 | 7 |
| EHBP1 | EH domain binding protein 1 | 7 |
| NEK4 | NIMA-related kinase 4 | 6 |
| PRKACB | Cyclic AMP (cAMP)-dependent protein kinase catalytic subunit | 5 |
| CHEK2 | Checkpoint kinase 2 | 3 |
| WDR79 | WD repeat domain 79 | 3 |
| RAB3B | Ras family member | 2 |
| TCF20 | Transcription factor 20 | 2 |
| RUNX3 | Runt-related transcription factor 3 | 1 |
| CHRM3 | Cholinergic receptor, muscarinic 3 | 1 |
| CDC2 | Cell division cycle 2 | 3 |
| CSNK1E | Casein kinase 1 epsilon | 2 |
| MCTP1 | Multiple C2 domains, transmembrane 1 | 1 |
| SNF1LK | SNF1-like kinase | 1 |
| FBXW7 | Fbox and WD40 domain protein 7 | 1 |
| KLF11 | Kruppel-like factor 11 | 1 |
| RAB14 | Ras family member | 1 |
a
Boldface, eliminated from follow-up due to low frequency of identification or previous experience with the genes or shRNAs.
b
The DNA obtained from pooled cultures was amplified by PCR and sequenced in bulk. For three of the five experimental pools (italics), a single shRNA was identified by this bulk-sequencing method. Amplified shRNAs from these pools were also cloned and sequenced, and the number of shRNAs identified for each gene is reported.