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. 2012 Oct;32(19):4001–4011. doi: 10.1128/MCB.00514-12

Fig 3.

Fig 3

S6K1−/− Akt2−/− mice have impaired insulin sensitivity. (A) Insulin tolerance test after intraperitoneal injection of insulin in 3-month-old (left) and 1-year-old (right) mice of the indicated genotypes (n ≥ 5). (B) RT-qPCR analyses of relative transcript levels of the indicated genes in the livers of 3-month old mice of the indicated genotypes using GUS as the reference gene and WT samples as the invariant control. Mice were starved for 16 h and refed for 4 h (n ≥ 5). (C) RT-qPCR analyses of relative transcript levels of GCK in hepatocytes kept in full medium 48 h after isolation from 2-month-old mice of the indicated genotypes using GUS as the reference gene and WT samples as the invariant control. (D) Immunoblot analysis of protein extracts from hepatocytes kept in full medium 48 h after isolation from 2-month-old mice of the indicated genotypes. The proteins were revealed using the indicated antibodies. The ratio to tubulin of the densitometric assay is presented. All values are expressed as means and SEM. a, P < 0.05 versus the wild-type group; b, P < 0.05 versus the S6K1−/− group; c, P < 0.05 versus the Akt2−/− group.