Skip to main content
. 2012 Oct;32(19):3938–3948. doi: 10.1128/MCB.00413-12

Fig 3.

Fig 3

C/EBPβ and STAT3 are crucial for IL-10-mediated NGAL expression. (a) A scheme showing point mutations in the respective putative binding sites of C/EBPβ and STAT3 in the NGAL promoter. (b and c) Luciferase reporter assay for the indicated full-length versus mutated NGAL promoter constructs after IL-10 stimulation for 3 h, where indicated in the presence of 10 μM Jak, STAT3 (STA-21), or PI3K (LY294002) and 5 μM p38(SB203580) inhibitors. Data are means ± SD; n = 8; ∗, P < 0.05 versus results for IL-10 and the bp −900 construct; #, P < 0.05 versus results for IL-10 with the bp −900 construct and C/EBPβ/STAT3. (d, e, and f) Oligonucleotides (3 μM) containing the binding sites for either STAT3 or C/EBPβ (de) or the mutated binding sites for each transcription factor (mt) were transfected separately in primary human macrophages prior to stimulation with IL-10 for 3 h. Scrambled oligonucleotides (sc) were used as controls. NGAL mRNA expression was measured by qRT-PCR. Data are means ± SD; n = 8; ∗, P < 0.05 versus results for IL-10.