Fig 1.

Kanamycin induces a self-limited increase in fecal water. Compared to those of untreated mice (hatched bar), fecal water contents of mice treated orally with kanamycin (open bars) were significantly higher for 48 h (P < 0.001; ANOVA with Dunnett's posttest modification). The number of days after kanamycin treatment is indicated under each open bar. We infected half of the mice with S. Typhimurium (WT) (closed bars) 48 h after the dose of kanamycin. The results for those mice are paired with the results for the mice receiving kanamycin alone for the first 2 days after infection. The difference in fecal water content of infected compared to kanamycin-treated control mice was also statistically significant for the 2 days after infection (***, P < 0.001; unpaired t test with Welch's correction).