Fig 2.

Fecal water content and weight loss after Salmonella infection. (A) Fecal water content was measured as described in Materials and Methods. There were 6 to 12 infected mice/group and 10 uninfected Kan-pretreated controls. Each bar shows the mean ± 1 standard error of the mean (SEM). Day 3 postinfection results were compared to results for controls 5 days after Kan treatment (***, P < 0.001). On day 7, we compared each mutant to the WT (*, P < 0.05; ***, P < 0.001). (B) Weight change, in grams, 7 days after infection. Each closed symbol represents an individual mouse infected orally, and the bars indicate the mean ± 1 SEM. There were only 4 mice in the orally infected WT group because 2 moribund mice had to be euthanized on day 6. Weight losses of mice infected i.p. with the WT are shown with open squares. All mutant bacterial infections except for those with the spvR mutant caused significantly less weight loss than WT infections (***, P < 0.001; ANOVA with Dunnett's posttest modification).